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Fig. 3 | BMC Medical Genomics

Fig. 3

From: A method for cryopreservation and single nucleus RNA-sequencing of normal adult human interventricular septum heart tissue reveals cellular diversity and function

Fig. 3

A method for cryopreserving and processing adult human heart tissue for single nuclei RNA-sequencing. a Workflow for cryopreserving adult human heart tissue and subsequent isolation and processing of nuclei for use in 10× Genomics microfluidics platform. b Demographics of the four unused donor hearts.cBatch-corrected Uniform Manifold Approximation and Projection (UMAP) of the integrated dataset. Each dot represents a single nucleus and is colored by cluster identity. Clusters are numbered in descending order of the number of nuclei assigned to that cluster. d Separated UMAP of each individual donor dataset demonstrating that every cell type was present in each donor and batch effects have been removed. e Heatmap of the top ten differentially expressed genes in each cluster across all the clusters. Each row is a different gene, colored by the expression level of that gene in each individual nucleus, with each column representing an individual nucleus. The expression of all of the top ten genes for each cluster across all clusters is displayed for each nucleus in the dataset. The nuclei are grouped by cluster, and the colored bar at the top indicates cluster number and corresponds to the colors in c

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