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Fig. 1 | BMC Medical Genomics

Fig. 1

From: Bioinformatics prediction of miR-30a targets and its inhibition of cell proliferation of osteosarcoma by up-regulating the expression of PTEN

Fig. 1

The level of PTEN and miR-30a is down-regulated in osteosarcoma cell lines.Transfection with miR-30a mimic or miR-30a inhibitor changes the relative level of miR-30a in MG63 cells. The osteosarcoma cells (MG63, 143B and Saos-2) were plated into a 48-well plate and cultured for 8 h. Primary osteoblasts were used as normal controls. a The levels of PTEN was determined by western blotting analysis. b The levels of relative expression of miR-30a was determined by real-time PCR assay. **p < 0.01, compared with osteoblasts

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