Fig. 3
From: A link between mitochondrial damage and the immune microenvironment of delayed onset muscle soreness
Screening and identification of hub genes. (A) Top: five overlapping genes with upregulated mitochondria (ARMCX1, AMPK, PGC1-α, SLC25A25, and TAMM41). The validation dataset (GSE23697) samples were divided into two groups of non-DOMS and DOMS patients, visualized using the R package ‘ggpubr’; p < 0.05 was set as significant, and one-way ANOVA was used. Bottom: real-time PCR using animal skeletal muscle samples. Data shown were normalized to GAPDH expression and were relative to expression in the non-DOMS (n = 3, error bars represent mean ± SD, *p < 0.05, and **p < 0.01 by Student’s t-test). (B) Spearman correlation was used to analyse the correlation between immune cells and hub genes, and a heat map was drawn. (C) The size of the lollipop corresponds to the strength of the correlation. (D) ARMCX1, AMPK, PGC1-α, and SLC25A25 correlation gene enrichment analysis