Fig. 4

Measurement of O2 consumption and immunofluorescence staining. (A) OCRs were quantified in adipose tissue under basal conditions (Basal) or with rotenone (Rot) disrupting the respiratory chain. Paired t test; *p < 0.05 and **p < 0.01. (B) ROS in frozen sections of human subcutaneous fat were stained by immunofluorescence. Nuclei were counterstained with DAPI. Fluorescence intensity was quantified using densitometric image analysis software with cell quantity adjustment. (C) Mitochondria in frozen sections of human subcutaneous fat were stained by immunofluorescence. Nuclei were counterstained with DAPI. Fluorescence intensity was quantified using densitometric image analysis software with cell quantity adjustment