Fig. 10

Validation of tumor-promoting effects of CYP19A1 in gastric cancer. Notes: A The expression level of CYP19A1 in several gastric cancer cell lines examined by qRT-PCR. B Knock-down and over-expression efficiencies of CYP19A1 were determined by qPCR and western blot. C-F CCK-8 assay was performed to explore the effect of proliferation of CYP19A1 in AGS and MKN45 cell lines. G and H The colony formation assay was used to validate the role CYP19A1 plays in the proliferation of AGS and MKN45 cell lines and the colonies were calculated. I and J The wound healing assay was used to explore the effect of migration of CYP19A1 in AGS and MKN45 cell lines and the rate of migration was calculated. K and L Transwell assays were used to validate the role CYP19A1 plays in the migration and invasion of AGS and MKN45 cell lines and the number of cells was calculated. M We carried out western blots and analyzed the expression of N-cadherin, E-cadherin, and vimentin to determine the correlation between CYP9A1 and EMT. The blots used in this figure conformed to the digital image and integrity policies. The blot and the corresponding internal reference in the same group were cropped from the same membrane and blots from different groups were from different membranes. The membrane was cut during the process of western blots according to the molecular mass of the target protein prior to hybridization with antibodies. One fuller-length, original, unprocessed blot performed with my samples for each antibody which confirms specific detection of the target antigen was provided in the supplementary material