Fig. 7

Effects of SERPINA3 knockdown on the colon cancer cell migration, proliferation and EMT. (A) HT29 and (B) SW620 cells were transfected with siRNAs targeting SERPINA3 or control siRNA, SERPINA3 expression was determined by evaluated by qRT-PCR. (C) HT29 and (D) SW620 cell caspase-3 activity was determined in different groups (si-NC, si- SERPINA3#1, si- SERPINA3#3). (E) HT29 and (F) SW620 cell caspase-9 activity was determined in different groups (si-NC, si- SERPINA3#1, si- SERPINA3#3). (G) HT29 and (H) SW620 cell proliferation was determined in different groups (si-NC, si- SERPINA3#1, si- SERPINA3#3). (I) HT29 and (J) SW620 cell migratory capacity was determined in different groups (si-NC, si- SERPINA3#1, si- SERPINA3#3). (K) HT29 and (L) SW620 cell EMT-related marker expression was evaluated in different groups (si-NC, si- SERPINA3#1, si- SERPINA3#3). N = 3. *P < 0.05, **P < 0.01 and ***P < 0.001