Fig. 8

SERPINA3 was negatively regulated by miR-137-3p and miR-296-5p. (A) HT29 cells were transfected with mimics-NC or mimics-miR-137-3p, miR-137-3p expression was determined by qRT-PCR. (B) Luciferase activity of wild-type or mutated reporter vector in different groups (NC, mimics-NC, mimics-miR-137-3p). (C) SERPINA3 expression was determined in different groups (mimics-NC, mimics-miR-137-3p). (D) HT29 cells were transfected with mimics-NC or mimics-miR-296-5p, miR-296-5p expression was determined by qRT-PCR. (E) Luciferase activity of wild-type or mutated reporter vector in different groups (NC, mimics-NC, mimics-miR-296-5p). (F) SERPINA3 expression was determined in different groups (mimics-NC, mimics-miR-296-5p). N = 3. “*P < 0.05, **P < 0.01 and ***P < 0.001”