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Table 1 Primer sequences used in minigene assay

From: Novel likely pathogenic variant in the EYA1 gene causing Branchio oto renal syndrome and the exploration of pathogenic mechanisms

Primer name

Primer sequence (5’- 3’)

EYA1-F

GCTCTGGAAACAGGCAAGAGG

EYA1-R

TGCAGTGTATGAATGCCGTTT

MINI-EYA1-BamH1-R

tagtGGATCCGGTGCTGCCATTCATTCTT

MINI-EYA1-Kpn1-F

ggtaGGTACCGCTCTATATGTGGTGCCAA

Exon8-Kpn-R

TAGTGGTACCCGGTGCTGCCATTCATTCTT

Exon8-Not-F

GGTAGCGGCCGCGAGCTCAGACTTGCAAAC

PcMINI-F

ctagagaacccactgcttac

PcMINI-R

tagaaggcacagtcgagg

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BMC Medical Genomics

ISSN: 1755-8794

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